Department of Molecular Immunology

Laboratory of Immunogenetics

Taranin Alexander
Head of the Department
Head of the Laboratory

Staff: 
thomson_logo.gif scopus_logo.jpg Taranin Alexander head DSc (Biology) taraninatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Volkova Olga senior research fellow PhD (Biology) volkovaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Mechetina Lyudmila senior research fellow PhD (Biology) lucieatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Najakshin Alexander senior research fellow PhD (Biology) najakshinatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Chikaev Nikolay senior research fellow PhD (Biology) na_chikatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Gorchakov Andrey senior research fellow PhD (Biology) gorchakovatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Baranov Konstantin senior research fellow PhD (Biology) baranovatmcb [dot] nsc [dot] ru
      Mayerova Anna engineer   maerovaatmcb [dot] nsc [dot] ru
      Starikovskaya Antonina lab technician   astarikovskaatmcb [dot] nsc [dot] ru
      Chernishova Zoya lab technician   z_chernishovaatmcb [dot] nsc [dot] ru
      Brusentsov Ilya part-time junior research fellow    
thomson_logo.gif scopus_logo.jpg   Solodkov Pavel part-time research assistant    
      Yurina Anna part-time research assistant    
      Murasheva Svetlana part-time research assistant    
      Shershneva Daria      

Former research workers:

Belovezhets Tatiana
Chernikova Daria
Chikaev Anton, PhD
Guselnikov Sergey, PhD
Istomina Polina
Khantakova Daria		 Kulemzin Sergey, PhD
Kuznetsova Valeria
Matvienko Daria
Reshetnikova Evdokia
Sizentsova Yana
Sokratyan Arpine

Research: 
EuroMabNet.png

The staff of the Laboratory are participants of EuroMAbNet - the European network of laboratories working in the field of monoclonal antibodies
Recent publications: 

  1. Belovezhets T, Kulemzin S, Volkova O, Najakshin A, Taranin A, Gorchakov A. Comparative pre-clinical analysis of CD20-specific CAR T cells encompassing 1F5-, Leu16-, and 2F2-based antigen-recognition moieties. (doi: 10.3390/ijms24043698) Int J Mol Sci 24(4): 3698, 2023

  2. Byazrova MG, Astakhova EA, Minnegalieva AR, Sukhova MM, Mikhailov AA, Prilipov AG, Gorchakov AA, Filatov AV. Anti-Ad26 humoral immunity does not compromise SARS-COV-2 neutralizing antibody responses following Gam-COVID-Vac booster vaccination. (doi: 10.1038/s41541-022-00566-x) npj Vaccines 7(1): 145, 2022

  3. Yusubalieva GM, Dashinimaev EB, Gorchakov AA, Kulemzin SV, Brovkina OA, Kalinkin AA, Vinokurov AG, Shirmanova MV, Taranin AV, Baklaushev VP. Enhanced natural killers with CISH and B2M gene knockouts reveal increased cytotoxicity in glioblastoma primary cultures. (doi: 10.1134/S0026893322050156) Mol Biology 56(5): 770-779, 2022

  4. Astakhova EA, Byazrova MG, Yusubalieva GM, Kulemzin SV, Kruglova NA, Prilipov AG, Baklaushev VP, Gorchakov AA, Taranin AV, Filatov AV. Functional profiling of in vitro reactivated memory B cells following natural SARS-CoV-2 infection and Gam-COVID-Vac vaccination. (doi: 10.3390/cells11131991) Cells 11(13): 1991, 2022

  5. Kulemzin SV, Sergeeva MV, Baranov KO, Gorchakov AA, Guselnikov SV, Belovezhets TN, Volkova OY, Najakshin AM, Chikaev NA, Danilenko DM, Taranin AV. VH3-53/66-Class RBD-specific human monoclonal antibody iB20 displays cross-neutralizing activity against emerging SARS-CoV-2 lineages. (doi: 10.3390/jpm12060895) J Pers Med 12(6): 895, 2022

  6. Jakovleva TV, Kazantseva AYu, Dubinina AD, Balybina NYu, Baranov KO, Makarova EN, Bazhan NM. Estradiol-dependent and independent effects of FGF21  in obese female mice. (doi: 10.18699/VJGB-22-20) Vavilov J Genet Breed 26(2): 159-168, 2022

  7. Zaboronok A, Khaptakhanova P, Uspenskii S, Bekarevich R, Mechetina L, Volkova O, Mathis BJ, Kanygin V, Ishikawa E, Kasatova A, Kasatov D, Shchudlo I, Sycheva T, Taskaev S, Matsumura A. Polymer-stabilized elemental boron nanoparticles for boron neutron capture therapy: Initial irradiation experiments. (doi: 10.3390/pharmaceutics14040761) Pharmaceutics 14(4): 761, 2022

  8. Bazhan SI, Antonets DV, Starostina EV, Ilyicheva TN, Kaplina ON, Marchenko VYu, Volkova OYu, Bakulina AYu, Karpenko LI. In silico design of influenza a virus artificial epitope-based T-cell antigens and the evaluation of their immunogenicity in mice. (doi: 10.1080/07391102.2020.1845978) J Biomol Struct Dyn 40(7): 3196-3212, 2022

  9. Borgoyakova MB, Karpenko LI, Rudometov AP, Volosnikova EA, Merkuleva IA, Starostina EV, Zadorozhny AM, Isaeva AA, Nesmeyanova VS, Shanshin DV, Baranov KO, Volkova NV, Zaitsev BN, Orlova LA, Zaykovskaya AV, Pyankov OV, Danilenko ED, Bazhan SI, Shcherbakov DN, Taranin AV, Ilyichev AA. Self-assembled particles combining SARS-CoV-2 RBD protein and RBD DNA vaccine induce synergistic enhancement of the humoral response in mice. (doi: 10.3390/ijms23042188) Int J Mol Sci 23(4): 2188, 2022

  10. Byazrova MG, Kulemzin SV, Astakhova EA, Belovezhets TN, Efimov GA, Chikaev AN, Kolotygin IO, Gorchakov AA, Taranin AV, Filatov AV. Memory B cells induced by Sputnik V vaccination produce SARS-CoV-2 neutralizing antibodies upon ex vivo restimulation. (doi: 10.3389/fimmu.2022.840707) Front Immunol 13: 840707, 2022

  11. Smagin DA, Kovalenko IL, Galyamina AG, Belozertseva IV, Tamkovich NV, Baranov KO, Kudryavtseva NN. Chronic lithium treatment affects anxious behaviors and the expression of serotonergic genes in midbrain raphe nuclei of defeated male mice. (doi: 10.3390/biomedicines9101293) Biomedicines 9(10): 1293, 2021

  12. Makarova E, Kazantseva A, Dubinina A, Jakovleva T, Balybina N, Baranov K, Bazhan N. The same metabolic response to FGF21 administration in male and female obese mice is accompanied by sex-specific changes in adipose tissue gene expression. (doi: 10.3390/ijms221910561) Int J Mol Sci 22(19): 10561, 2021

  13. Makarova E, Kazantseva A, Dubinina A, Denisova E, Jakovleva T, Balybina N, Bgatova N, Baranov K, Bazhan N. Fibroblast growth factor 21 (FGF21) administration sex-specifically affects blood insulin levels and liver steatosis in obese Ay mice. (doi: 10.3390/cells10123440) Cells 10(12): 3440, 2021

  14. Kulemzin S, Evsyukov I, Belovezhets T, Taranin A, Gorchakov A. Horses for courses in the era of CARs: Advancing CAR T and CAR NK cell therapies. (doi: 10.3390/jpm11111182) J Pers Med 11(11): 1182, 2021

  15. Gorchakov AA, Kulemzin SV, Guselnikov SV, Baranov KO, Belovezhets TN, Mechetina LV, Volkova OYu, Najakshin AM, Chikaev NA, Chikaev AN, Solodkov PP, Larichev VF, Gulyaeva MA, Markhaev AG, Kononova YuV, Alekseyev AYu, Shestopalov AM, Yusubalieva GM, Klypa TV, Ivanov AV, Valuev-Elliston VT, Baklaushev VP, Taranin AV. Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern. (doi: 10.1038/s41421-021-00340-8) Cell Discov 7: 96, 2021

  16. Zaboronok A, Taskaev S, Volkova O, Mechetina L, Kasatova A, Sycheva T, Nakai K, Kasatov D, Makarov A, Kolesnikov I, Shchudlo I, Bykov T, Sokolova E, Koshkarev A, Kanygin V, Kichigin A, Mathis BJ, Ishikawa E, Matsumura A. Gold nanoparticles permit in situ absorbed dose evaluation in boron neutron capture therapy for malignant tumors. (doi: 10.3390/pharmaceutics13091490) Pharmaceutics 13(9): 1490, 2021

  17. Smagina AS, Kulemzin SV, Yusubalieva GM, Kedrova AG, Sanzharov AE, Ivanov YV, Matvienko DA, Kalsin VA, Gorchakov AA, Baklaushev VP, Taranin AV. VAV1‐overexpressing YT cells display improved cytotoxicity against malignant cells. (doi: 10.1002/bab.2001) Biotechnol Appl Biochem 68(4): 849-855, 2021

  18. Starostina EV, Sharabrin SV, Antropov DN, Stepanov GA, Shevelev GYu, Lemza AE, Rudometov AP, Borgoyakova MB, Rudometova NB, Marchenko VYu, Danilchenko NV, Chikaev AN, Bazhan SI, Ilyichev AA, Karpenko LI. Construction and immunogenicity of modified mRNA-vaccine variants encoding influenza virus antigens (doi: 10.3390/vaccines9050452) Vaccines 9(5): 452, 2021

  19. Byazrova M, Yusubalieva G, Spiridonova A, Efimov G, Mazurov D, Baranov K, Baklaushev V, Filatov A. Pattern of circulating SARS-CoV-2-specific antibody-secreting and memory B-cell generation in patients with acute COVID-19. (doi: 10.1002/cti2.1245) Clin Transl Immunology 10(2): e1245, 2021

  20. Uspenskii SA, Khaptakhanova PA, Zaboronok AA, Kurkin TS, Volkova OYu, Mechetina LV, Taranin AV, Kanygin VV, Matsumura A, Taskaev SYu. Elemental boron nanoparticles: production by ultrasonication in aqueous medium and application in boron neutron capture therapy. (doi: 10.1134/S0012500820030027) Dokl Chem 491: 45-48, 2020

  21. Gorchakov AA, Kulemzin SV, Kochneva GV, Taranin AV. Challenges and prospects of chimeric antigen receptor T-cell therapy for metastatic prostate cancer. (doi: 10.1016/j.eururo.2019.08.014) Eur Urol 77(3): 299-308, 2020

  22. Kochneva GV, Sivolobova GF, Tkacheva AV, Gorchakov AA, Kulemzin SV. Combination of oncolytic virotherapy and CAR T/NK cell therapy for the treatment of cancer. (doi: 10.1134/S0026893320010100) Mol Biol 54(1): 1-12, 2020

  23. Shcherbakova NS, Chikaev AN, Rudometov AP, Shcherbakov DN, Il’ichev AA, Karpenko LI. Characteristics of artificial immunogens containing peptide mimotopes of HIV-1 epitopes recognized by monoclonal antibodies 2F5 and 2G12. (doi: 10.1007/s10517-019-04504-1) Bull Exp Biol Med 167(2): 259-262, 2019

  24. Kulemzin SV, Gorchakov AA, Taranin AV. Prostate cancer surface targets for CAR T cell therapy or metastatic prostate cancer in the CAR T cell era: My kingdom for the target! (doi: 10.18620/ctt-1866-8836-2019-8-4-19-28) Cell Ther Transplant 8(4): 19-28, 2019

  25. Guselnikov SV, Belovezhets TN, Kulemzin SV, Gorchakov AA, Taranin AV. A simple way to increase recovery of the expressed VH and VL genes in single-sorted human B cells.  (doi: 10.2144/btn-2019-0079) BioTechniques 67(4): 184-187, 2019

  26. Rudometov AP, Chikaev AN, Rudometova NB, Antonets DV, Lomzov AA, Kaplina ON, Ilyichev AA, Karpenko LI. Artificial anti-HIV-1 immunogen comprising epitopes of broadly neutralizing antibodies 2F5, 10E8, and a peptide mimic of VRC01 discontinuous epitope. (doi: 10.3390/vaccines7030083) Vaccines 7(3): 83, 2019

  27. Shcherbakova NS, Chikaev AN, Rudometov AP, Shcherbakov DN, Il’ichev AA, Karpenko LI. Characteristics of artificial immunogens containing peptide mimotopes of HIV-1 epitopes recognized by monoclonal antibodies 2F5 and 2G12. (doi: 10.1007/s10517-019-04504-1) Bull Exp Biol Med 167(2): 259-262, 2019 

  28. Guselnikov SV, Taranin AV. Unraveling the LRC evolution in mammals: IGSF1 and A1BG provide the keys. (doi: 10.1093/gbe/evz102) Genome Biol Evol 11(6): 1586-1601, 2019

  29. Kulemzin SV, Matvienko DA, Sabirov AH, Sokratyan AM, Chernikova DS, Belovezhets TN, Chikaev AN, Taranin AV, Gorchakov AA. Design and analysis of stably integratedreporters for inducible transgeneexpression in human T cells and CAR NK-cell lines. (doi: 10.1186/s12920-019-0489-4) BMC Med Genom 12 (Suppl 2): 44, 2019

  30. Copur Ö, Gorchakov A, Finkl K, Kuroda MI, Müller J. Sex-specific phenotypes of histone H4 point mutants establish dosage compensation as the critical function of H4K16 acetylation in Drosophila. (doi: 10.1073/pnas.1817274115) Proc Natl Acad Sci USA 115(52): 13336-13341, 2018

  31. Matvienko DA, Kulemzin  SV, Smagina AS, Belovezhets TN, Chikaev AN, Volkova OY, Chikaev NA, Koval OA, Kuligina EV, Taranin AV, Gorchakov AA. Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. (doi: 10.18620/ctt-1866-8836-2018-7-2-70-77) Cell Therapy Transplantation (CTT) 7(2): 70-77, 2018

  32. Reshetnikova E, Guselnikov S, Volkova O, Baranov K, Taranin A, Mechetina L. B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. (doi: 10.1002/cyto.b.21611) Cytometry B Clin Cytom 94(4): 549-704, 2018

  33. Gorchakov AA, Sizentsova YaG, Belovezhets TN, Kulemzin SV, Chikaev AN, Guselnikov SV, Taranin AV. Design and functional analysis of Fn3-based bi-specific cars. (doi: 10.1016/j.ymthe.2018.05.001) Mol Therapy 26 (5 S1): 60, 2018

  34. Gorchakov A, Chikaev AN, Kulemzin SV, Volkova OY, Chernikova DS, Matvienko DA, Belovezhets TN, Sokratyan AM, Kuznetsova VV, Taranin AV. ECAR-NK cell platform: enhancing the CAR-mediated NK cell cytotoxicity via blockade of the CD47/SIRPa axis. (doi: 10.1016/j.ymthe.2018.05.001) Mol Therapy 26 (5 S1): 199, 2018

  35. Guselnikov SV, Baranov KO, Najakshin AM, Mechetina LV, Chikaev NA, Makunin AI, Kulemzin SV, Andreyushkova DA, Stöck M, Wuertz S, Gessner J, Warren WC, Schartl M,  Trifonov VA, Taranin AV. Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes. (doi: 10.3389/fimmu.2018.01079) Front Immunol 9: 1079, 2018

  36. Sato E, Zaboronok A, Yamamoto T, Nakai K, Taskaev S, Volkova O, Mechetina L, Taranin A, Kanygin V, Isobe T, Mathis BJ, Matsumura A. Radiobiological response of U251MG, CHO-K1 and V79 cell lines to accelerator-based boron neutron capture therapy. (doi: 10.1093/jrr/rrx071) J Radiat Res 59(2): 101-107, 2018

  37. Kulemzin SV, Gorchakov AA, Chikaev AN, Kuznetsova VV, Volkova OY, Matvienko DA, Petukhov AV, Zaritskey AY, Taranin AV. VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells. (doi: 10.18632/oncotarget.24078) Oncotarget 9(10): 9021-9029, 2018

  38. Koval O, Volkova O, Kulemzin S, Gorchakov A, Tkachenko A, Nushtaeva A, Kuligina E, Richter V, Taranin A. NK-cell based delivery of anticancer therapeutics. (doi: 10.1093/annonc/mdx711.050) Annals Oncology 28(Suppl 11): 69P, 2017

  39. Gorchakov A, Kulemzin S, Kuznetsova V, Volkova O, Taranin A. FnCARs: a platform expanding the repertoire of proteins targetable with chimeric antigen receptors. Mol Therapy 25(5S1): 272, 2017

  40. Zaboronok AA, Byvaltsev VA, Kanygin VV, Iarullina AI, Kichigin AI, Taranin AV, Volkova OYu, Mechetina LV, Taskaev SYu, Muhamadiyarov RA, Zavyalov EL, Nakai K, Sato E, Yamamoto T, Mathis BJ, Matsumura A. Boron-neutron capture therapy in Russia: preclinical evaluation of efficacy and perspectives of its application in neuro-oncology. New Armenian Med J 11(1): 6-15, 2017

  41. Sherin PS, López-Duarte I, Dent MR, Kubánková M, Vyšniauskas A, Bull JA, Reshetnikova ES, Klymchenko AS, Tsentalovich YP, Kuimova MK. Visualising the membrane viscosity of porcine eye lens cells using molecular rotors. (doi: 10.1039/C6SC05369F) Chem Sci 8: 3523-3528, 2017

  42. Kulemzin SV, Kuznetsova VV, Mamonkin M, Taranin AV, Gorchakov AA. CAR T-cell therapy: Balance of efficacy and safety. Mol Biology 51(2): 237-250, 2017

  43. Kulemzin SV, Chikaev NA, Volkova OY, Kuznetsova VV, Taranin AV, Gorchakov AA. Modular lentiviral vector system for chimeric antigen receptor design optimization. Rus J Bioorg Chem 43(2): 107-114, 2017

  44. Kulemzin SV, Kuznetsova VV, Mamonkin M, Taranin AV, Gorchakov АA. Engineering chimeric antigen receptors. Acta Naturae 9(1): 6-14, 2017

  45. Sokratian A, Baranov K, Guselnikov S, Chikaev N, Volkova O, Mechetina L, Taranin A, Gorchakov A , Kulemzin S. Identification of the human SLAMF9-receptor complex. Tissue Antigens 85: 381-382, 2015

  46. Guselnikov SV, Grayfer L, De Jesús Andino F, Rogozin IB, Robert J, Taranin AV. Retention of duplicated ITAM-containing transmembrane signaling subunits in the tetraploid amphibian species Xenopus laevis. (doi: 10.1016/j.dci.2015.07.002) Dev Comp Immunol 53: 158-168, 2015

  47. Romanets-Korbut O, Najakshin AM, Yurchenko M, Malysheva TA, Kovalevska L, Shlapatska LM, Zozulya YA, Taranin AV, Horvat B, Sidorenko SP. Expression of CD150 in tumors of the central nervous system: identification of a novel isoform. (doi: 10.1371/journal.pone.0118302) PLoS One 10(2): e0118302, 2015

  48. Reguzova AY, Karpenko LI, Mechetina LV, Belyakov IM. Peptide–MHC multimer-based monitoring of CD8 T-cells in HIV-1 infection and AIDS 5 vaccine development. (doi: 10.1586/14760584.2015.962520) Expert Rev Vaccines 14: 69-84, 2015

Selected talks: 

  1. Gorchakov A. Adnectin-based CARs: pros and cons. XII Raisa Gorbacheva Memorial International Symposium "Hematopoietic Stem Cell Transplantation. Gene and Cellular Therapy", 20-22 September 2018, St. Petersburg, Russia

  2. Chikaev A. CD47/SIRPa blockers and CARs: together we stand. XII Raisa Gorbacheva Memorial International Symposium "Hematopoietic Stem Cell Transplantation. Gene and Cellular Therapy", 20-22 September 2018, St. Petersburg, Russia

  3. Belovezhets T. Quest for the optimal NK-cell line as a CAR carrier. XII Raisa Gorbacheva Memorial International Symposium "Hematopoietic Stem Cell Transplantation. Gene and Cellular Therapy", 20-22 September 2018, St. Petersburg, Russia

  4. Kulemzin S. Forging the Natural Killer with genome editing. XII Raisa Gorbacheva Memorial International Symposium "Hematopoietic Stem Cell Transplantation. Gene and Cellular Therapy", 20-22 September 2018, St. Petersburg, Russia

  5. Kulemzin SV. Generation of NK-cell lines with enhanced therapeutic potential. International congress “CRISPR-2018”, September 10-14, 2018, Novosibirsk, Russia

  6. Matvienko D. PSMA- and PSCA-specific chimeric antigen receptors (CARs) for prostate cancer therapy. The 25th International Students Congress of bio(Medical) Science (ISCOMS 2018) 4-8 June 2018, Groningen, Netherlands

  7. Gorchakov A. Optimal CAR design and development of an ECAR NK cell platform. XI Raisa Gorbacheva Memorial Meeting “Hematopoetic Stem Cell Transplantation. Gene and Cellular Therapy”, Workshop on Genome Editing in Hematology & HSCT, 14-16 September 2017, St. Petersburg, Russia

  8. Mechetina L. Artificial antibodies based on human 10Fn3 scaffold. 8th EuroMAbNet Meeting, 22-23 September 2016, Opatija, Croatia

  9. Baranov K. Multimeric 10Fn3-based proteins as inductors of anti-idiotypic antibodies. 8th EuroMAbNet Meeting, 22-23 September 2016, Opatija, Croatia

  10. Taranin AV. Dot immunoenzyme assay for the diagnosis of Aleutian mink disease. XIth International Scientific Congress in Fur Animal Production. 23-26 August 2016, Helsinki, Finland

  11. Mechetina LV. New intacellular B cell marker FCRLA. 5th EuroMabNet meeting, 23-24 September 2013, Menorca, Spain

  12. Mechetina L. FCRL6 receptor: expression and associated proteins. 9th International Conference on Human Leukocyte Differentiation Antigens. 11-13 March 2010, Barcelona, Spain

  13. Baranov KO. Searching for ligands of the FcR-like B cell-specific receptors. 9th International Conference on Human Leukocyte Differentiation Antigens. 11-13 March 2010, Barcelona, Spain

  14. Taranin A. Molecular Evolution of the FcR- and KIR-like Receptors in Tetrapods. ISDCI 10th International Congress,
    1-6 July 2006, Charleston, USA

  15. Guselnikov S. Evolution of FcR- and KIR-like leukocyte receptors: from Teleostei to Mammalia. Eastern Great Lakes Molecular Evolution Meeting. Koffler Institute for Pharmacy Management, University of Toronto, 30 April 2005, Toronto, Canada

Collaboration: 

  • University of Alabama at Birmingham, AL, USA

  • University of Rochester Medical Center, NY, USA

  • Universitat de Barcelona, Spain

  • Faculty of Medicine, University of Tsukuba, Japan

Research Groups

Department of Structure and Function of Chromosomes
Department of Diversity and Evolution of Genomes
Department of Regulation of Genetic Processes