Department of Structure and Function of Chromosomes

Laboratory of Molecular Genetics

Vershinin Alexander
Head of the Laboratory

Staff: 
thomson_logo.gif scopus_logo.jpg Vershinin Alexander head DSc (Biology) avershinatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg   Evtushenko Elena senior research fellow PhD (Biology) evtatmcb [dot] nsc [dot] ru
thomson_logo.gif     Terletsky Alexander research fellow PhD (Biology) terletskyatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg   Gatskaya Sima junior research fellow  
      Trunin Sergey research assistant    
  scopus_logo.jpg   Titov Sergey part-time senior research fellow PhD (Biology)  
thomson_logo.gif scopus_logo.jpg   Veryaskina Yulia part-time senior research fellow PhD (Biology) juliaveratmcb [dot] nsc [dot] ru
      Elisaphenko Evgeny part-time research fellow PhD (Biology)  
  scopus_logo.jpg   Ivanov Mikhail part-time research fellow PhD (Biology)  
      Kovynev Igor part-time research fellow PhD (Biology)  
      Malakhina Ekaterina part-time research assistant PhD (Medicine)  
      Klimova Olesya part-time research assistant    
      Maslov Konstantin part-time research assistant    
      Novozhilov Nikolay part-time research assistant    

Former research workers:

Gaskov Dmitry
Grishko Ekaterina
Gunbin Konstantin, PhD
Kolesnikov Nikolay, DSc
Lipikhina Yulia
Serdyukova Olga

Recent publications: 
  1. Lukyanov SA, Sergiyko SV, Titov SE, Beltsevich DG, Veryaskina YA, Vanushko VE, Urusova LS, Mikheenkov AA, Kozorezova ES, Vorobyov SL, Sleptsov IV. New opportunities for preoperative diagnosis of medullary thyroid carcinoma. (doi: 10.3390/biomedicines11051473) Biomedicines 11(5): 1473, 2023

  2. Ivanov MK, Brenner EV, Hodkevich AA, Dzyubenko VV, Krasilnikov SE, Mansurova AS, Vakhturova IE, Agletdinov EF, Shumeikina AO, Chernyshova AL, Titov SE. Cervicovaginal-microbiome analysis by 16S sequencing and Real-Time PCR in patients from Novosibirsk (Russia) with cervical lesions and several years after cancer treatment. (doi: 10.3390/diagnostics13010140) Diagnostics 13(1): 140, 2023

  3. Veryaskina YA, Titov SE, Kovynev IB, Pospelova TI, Fyodorova SS, Shebunyaeva YY, Sumenkova DV, Zhimulev IF. MicroRNA expression profile in bone marrow and lymph nodes in B-cell lymphomas. (doi: 10.3390/ijms242015082) Int J Mol Sci 24(20): 15082, 2023

  4. Vershinin AV, Elisafenko EA, Evtushenko EV. Genetic redundancy in rye shows in a variety of ways. (doi: 10.3390/plants12020282Plants 12(2): 282, 2023

  5. Stupak EV, Veryasikina YA, Titov SE, Askandaryan AS, Hiana JC, Zhimulyov IF, Stupak VV. MicroRNAs in the diagnosis of malignancy of supratentorial brain gliomas and prognosis of disease progression. (doi: 10.7759/cureus.35906) Cureus 15(3): e35906, 2023

  6. Mikhailova AG, ... Gunbin K, Popadin K. A mitochondria-specific mutational signature of aging: increased rate of A > G substitutions on the heavy strand (doi: 10.1093/nar/gkac779) Nucleic Acids Res 50(18): 10264-10277, 2022

  7. Kazachkov EL, Semenov YuA, Veryaskina YuA, Kazachkova EA, Chizhovskaya AV. The expression profi le of a number of miRNAs in the placenta in timely and premature birth. (doi: 10.31549/2542-1174-2022-6-1-22-37) Journal of Siberian Medical Sciences 6(1): 22-37, 2022

  8. Veryaskina YA, Titov SE, Zhimulev IF. Reference genes for qPCR-based miRNA expression profiling in 14 human tissues. (doi: 10.1159/000524283) Med Princ Pract 31: 322-332, 2022

  9. Ryabinin VA, Akimov IA, Sinyakov AN, Pichko NP, Ivanov MK. Hepatitis C virus RF1_2k/1b chimeras and a “Many probes/one spot” approach to their identification. (doi: 10.1134/S1068162022010095) Rus J Bioorg Chem 48(1): 173-177, 2022

  10. Veryaskina YuA, Titov SE, Ivanov MK, Ruzankin PS, Tarasenko AS, Shevchenko SP, Kovynev IB, Stupak EV, Pospelova TI, Zhimulev IF. Selection of reference genes for quantitative analysis of microRNA expression in three different types of cancer. (doi: 10.1371/journal.pone.0254304PLoS ONE 17(2): e0254304, 2022

  11. Elisafenko EA, Evtushenko EV, Vershinin AV. The origin and evolution of a two-component system of paralogous genes encoding the centromeric histone CENH3 in cereals. (doi: 10.1186/s12870-021-03264-3BMC Plant Biol 21: 541, 2021

  12. Vershinin AV, Lux T, Gundlach H, Elisafenko EA, Keilwagen J, Mayer KFX, Spannagl M. The gene and repetitive element landscape of the rye genome. In: "The Rye Genome" (Rabanus-Wallace MT, Stein N, Eds), Springer, pp 117-133, 2021 (doi: 10.1007%2F978-3-030-83383-1_8)

  13. Evtushenko EV, Elisafenko EA, Gatzkaya SS, Schubert V, Houben A, Vershinin AV. Expression of two rye CENH3 variants and their loading into centromeres. (doi: 10.3390/plants10102043Plants 10(10): 2043, 2021

  14. Ivanov MK, Titov SE, Dzyubenko VV, Glushkov SA, Krasilnikov SE, Mansurova AS, Malek AV, Berlev IV, Prisyazhnaya TS, Kuleshova SV, Hodkevich AA, Lancuhaj YA, Dimitriadi TA, Agletdinov EF. Detection of cervical lesions and cancer in air-dried cytologic smears by combined analysis of mRNA and miRNA expression levels. (doi: 10.1016/j.jmoldx.2021.01.016J Mol Diagn 23(5): 541-554, 2021

  15. Titov SE, Poloz TL, Veryaskina YA, Anishchenko VV. Cytological and molecular diagnosis of Hürthle cell thyroid tumors: Analysis of three cases. (doi: 10.3892/mco.2021.2311Mol Clin Oncol 15(1): 149, 2021

  16. Konnikova MR, Cherkasova OP, Nazarov MM, Vrazhnov DA, Kistenev YV, Titov SE, Kopeikina EV, Shevchenko SP, Shkurinov AP. Malignant and benign thyroid nodule differentiation through the analysis of blood plasma with terahertz spectroscopy. (doi: 10.1364/BOE.412715Biomed Opt Express 12(2): 1020-1035, 2021

  17. Veryaskina YA, Titov SE, Kovynev IB, Fedorova SS, Pospelova TI, Zhimulev IF. MicroRNAs in the myelodysplastic syndrome. (doi: 10.32607/actanaturae.11209Acta Naturae 13(2): 4-15, 2021

  18. Titov SE, Kozorezova ES, Demenkov PS, Veryaskina YA, Kuznetsova IV, Vorobyev SL, Chernikov RA, Sleptsov IV, Timofeeva NI, Ivanov MK. Preoperative typing of thyroid and parathyroid tumors with a combined molecular classifier. (doi: 10.3390/cancers13020237Cancers 13(2): 237, 2021

  19. Kovynev IB, Titov SE, Ruzankin PS, Agakishiev MM, Veryaskina YA, Nedel’ko VM, Pospelova TI, Zhimulev IF. Profiling 25 bone marrow microRNAs in acute leukemias and secondary nonleukemic hematopoietic conditions. (doi: 10.3390/biomedicines8120607Biomedicines 8(12): 607, 2020

  20. Ellis THN, Vershinin AV. Retrotransposons and the evolution of genome size in Pisum. (doi: 10.3390/biotech9040024) BioTech 9(4): 24, 2020

  21. Titov S, Demenkov PS, Lukyanov SA, Sergiyko SV, Katanyan GA, Veryaskina YA, Ivanov MK. Preoperative detection of malignancy in fine-needle aspiration cytology (FNAC) smears with indeterminate cytology (Bethesda III, IV) by a combined molecular classifier. (doi: 10.1136/jclinpath-2020-206445J Clin Pathol 73(11): 722-727, 2020

  22. Koroleva AG, Evtushenko EV, Vershinin AV, Zaytseva EP, Timoshkin OA, Kirilchik SV. Age dynamics of telomere length in endemic Baikal planarians (doi: 10.1134/S002689332004007X) Mol Biol 54(4): 553-562, 2020

  23. Veryaskina YuA, Titov SE, Kovynev IB, Pospelova TI, Zhimulev IF. The miRNA profile in non-Hodgkin’s lymphoma patients with secondary myelodysplasia. (doi: 10.3390/cells9102318Cells 9(10): 2318, 2020

  24. Veryaskina YuA, Titov SE, Kovynev IB, Pospelova TI, Zhimulev IF. Prognostic markers of myelodysplastic syndromes. (doi: 10.3390/medicina56080376Medicina 56(8): 376, 2020

  25. Veryaskina YuA, Titov SE, Kometova VV, Rodionov VV, Zhimulev IF. Intratumoral heterogeneity of expression of 16 miRNA in luminal cancer of the mammary gland. (doi: 10.3390/ncrna6020016Non-Coding RNA 6(2): 16, 2020

  26. Evtushenko EV, Lipikhina YuA, Stepochkin PI, Vershinin AV. Cytogenetic and molecular characteristics of rye genome in octoploid triticale (× Triticosecale Wittmack). (doi: 10.3897/CompCytogen.v13i4.39576Comp Cytogenet 13(4): 423-434, 2019

  27. Titov SE, Ivanov MK, Demenkov PS, Katanyan GA, Kozorezova ES, Malek AV, Veryaskina YuA, Zhimulev IF. Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears. (doi: 10.1186/s12885-019-6154-7BMC Cancer 19: 1010, 2019

  28. Kolesnikov NN, Veryaskina YuA, Titov SE, Rodionov VV, Gening TP, Abakumova TV, Kometova VV, Torosyan MKh, Zhimulev IF. Expression of micrornas in molecular genetic breast cancer subtypes. (doi: 10.1016/j.ctarc.2016.08.006) Cancer Treat Res Commun 20: 100026, 2019

  29. Terletsky AV, Akhmerova LG, Evtushenko EV. Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland. (doi: 10.15789/2220-7619-2019-1-155-161) Rus J Infection Immunity 9(1): 155-161, 2019

  30. Gatzkaya SS, Evtushenko EV. Patterns of nucleotide diversity for different domains of centromeric histone H3 (CENH3) gene in Secale L. (doi: 10.18699/VJ19.472) Vavilov J Genetics Breeding 23(2): 135-139, 2019

  31. Serdyukova OS, Titov SE, Malakhina ES, Rymar OD. MicroRNAs – promising molecular markers for detecting cancer in thyroid nodules. (doi: 10.14341/ket9774) Clinical and experimental thyroidology 14(3): 140-148, 2018 (Abstract in English)

  32. Lipikhina YuA, Evtushenko EV, Lyusikov OM, Gordei IS, Gordei IA, Vershinin AV. Dynamics of the centromeric histone CENH3 structure in rye-wheat amphidiploids (Secalotriticum). (doi: 10.1155/2018/2097845BioMed Res Int 2018: 2097845, 2018

  33. Ovsyannikova AK, Rymar OD, Ivanoshchuk DE, Mikhailova SV, Shakhtshneider EV, Orlov PS, Malakhina ES, Voevoda MI. A case of maturity onset diabetes of the young (MODY3) in a family with a novel HNF1A gene mutation in five generations. (doi: 10.1007/s13300-017-0350-8Diabetes Ther 9(1): 413-420, 2018

  34. Ivanov MK, Titov SE, Glushkov SA, Dzyubenko VV, Malek AV, Arkhangelskaya PA, Samsonov RB, Mikhetko AA, Bakhidze EV, Berlev IV, Kolesnikov NN. Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier. (doi: 10.3892/or.2018.6214Oncology Rep 39: 1099-1111, 2018 

  35. Lipikhina YA, Evtushenko EV, Elisafenko EA, Vershinin AV. Chromosomal assignment of centromere-specific histone CENH3 genes in rye (Secale cereale L.) and their phylogeny. (doi: 10.3897/CompCytogen.v11i4.19953Comp Cytogenet 11(4): 821-832, 2017

  36. Evtushenko EV, Elisafenko EA, Gatzkaya SS, Lipikhina YA, Houben A, Vershinin AV. Conserved molecular structure of the centromeric histone CENH3 in Secale and its phylogenetic relationships. (doi: 10.1038/s41598-017-17932-8Sci Reports 7: 17628, 2017

  37. Stolyar M, Klimova O, Ivanov M, Gorbenko A, Titov S, Olkhovskiy I. JAK2 haplotype 46/1 (GGCC) has no effect on the primary risk of JAK2 V617F mutation, but it strongly potentiates the progression of grown allele burden in myeloproliferative neoplasms. Haematologica 102(s2): 539, 2017

  38. Malek A, Sleptsov I, Cheburkin Y, Samsonov R, Kolesnikov N. miRNA as potential tool for thyroid cancer diagnostics and follow up: practical considerations. JSM Thyroid Disord Manag 2(1): 1007, 2017

  39. Boldyreva LV, Goncharov FP, Demakova OV, Zykova TYu, Levitsky VG, Kolesnikov NN, Pindyurin AV, Semeshin VF, Zhimulev IF. Protein and genetic composition of four chromatin types in Drosophila melanogaster cell lines. (doi:  10.2174/1389202917666160512164913Curr Genomics, 18(2): 214-226, 2017

  40. Stupak EV, Veryaskina YuA, Titov SE, Achmerova LG, Stupak VV, Ivanov MK, Zhimulev IF, Kolesnikov NN. MicroRNA profiling in the malignant progression of gliomas. AIP Conf Proc 1760, 020065, doi: 10.1063/1.4960284, 2016

  41. Evtushenko  EV, Levitsky VG, Elisafenko EA, Gunbin KV, Belousov AI, Šafář J, Doležel J, Vershinin AV. The molecular mechanisms of heterochromatin expansion in rye chromosomes. Cytogenet Genome Res 148: 91, 2016

  42. Titov SE, Demenkov PS, Ivanov MK, Malakhina ES, Poloz TL, Tsivlikova EV, Ganzha MS, Shevchenko SP, Gulyaeva LF, Kolesnikov NN. Selection and validation of miRNAs as normalizers for profiling expression of microRNAs isolated from thyroid fine needle aspiration smears. (doi: 10.3892/or.2016.5113Oncology Rep 36: 2501-2510, 2016

  43. Samsonov R, Burdakov V, Shtam T, Radzhabovа Z, Vasilyev D, Tsyrlina E, Titov S, Ivanov M, Berstein L, Filatov M, Kolesnikov N, Gil-Henn H, Malek A. Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer. (doi: 10.1007/s13277-016-5065-3Tumor Biol 37: 12011-12021, 2016

  44. Evtushenko EV, Levitsky VG, Elisafenko EA, Gunbin KV, Belousov AI, Šafář J, Doležel J, Vershinin AV. The expansion of heterochromatin blocks in rye reflects the co-amplification of tandem repeats and adjacent transposable elements. (doi: 10.1186/s12864-016-2667-5BMC Genomics 17(1): 337, 2016

  45. Titov SE, Ivanov MK, Karpinskaya EV, Tsivlikova EV, Shevchenko SP, Veryaskina YA, Akhmerova LG, Poloz TL, Klimova OA, Gulyaeva LF, Zhimulev IF, Kolesnikov NN. miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors. (doi: 10.1186/s12885-016-2240-2BMC Cancer 16: 201, 2016

  46. Shevchenko S, Mostovich L, Kolesnikov N, Gulyaeva L. Molecular biomarkers in preoperative diagnosis of thyroid cancer. Eur J Cancer Suppl 13(1): 52, 2015

Selected talks: 
  1. Evtushenko EV. Evolutionary dynamics of the centomeric histone CENH3 in the Triticeae tribe. International Conference “Chromosome – 2018”, 20-24 August 2018, Novosibirsk, Russia

  2. Vershinin A. The molecular mechanisms of the heterochromatin expansion in the rye chromosomes. 21st International Chromosome Conference. July 10-13, 2016, Foz do Iguaçu, Brazil

  3. Vershinin AV. Intensive recombinations have led to tandem repeat expansion and enlargement of rye subtelomeric heterochromatin. The 19th International Chromosome Conference. 2-6 September 2013, Bologna, Italy

  4. Vershinin AV. Genomics of rye subtelomeric heterochromatin. 11th Gatersleben Research Conference "Chromosome biology, genome evolution, and speciation" 23-25 April 2012, Gatersleben, Germany

  5. Vershinin AV. The genomics of the most abundant tandem repeat DNA families in the rye heterochromatin. Annual Main Meeting of the Society for Experimental Biology. Chromosome Organization and Cytogenomics, 30 June - 3 July 2010, Prague, Czech Republic

  6. Kolesnikov NN. X-chromosome inactivation, mobile elements and ncRNA genes. 6th International Conference on Bioinformatics of Genome Regulation and Structure, 22-28 June 2008, Novosibirsk, Russia

  7. Kolesnikov NN. Evolution of the structure of the XIST locus in mammals. 5th International Conference on Bioinformatics of Genome Regulation and Structure, 16-22 July 2006, Novosibirsk, Russia

Collaboration: 
  • Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany

  • Institute of Experimental Botany, Olomouc, Czech Republic

  • Institute of Genetics and Cytology, Minsk, Belarus

  • John Innes Centre, Norwich, UK