Department of the Regulation of Genetic Processes

Laboratory of Cell Division

Pindyurin Alexey
Head of the Laboratory

Curriculum vitae

Staff: 
thomson_logo.gif scopus_logo.jpg Pindyurin Alexey head PhD (Biology) a [dot] pindyurinatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Andreyeva Evgeniya senior research fellow PhD (Biology) andreevaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Boldyreva Lidiya senior research fellow PhD (Biology) asdatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Omelina Evgeniya senior research fellow PhD (Biology) omelinaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Ogienko Anna research fellow PhD (Biology) ogienko [dot] annaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Kopyl Sergei research fellow PhD (Biology) kopylatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Dubatolova Tatiana research fellow   tdubatatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Yarinich Lyubov junior research fellow   l [dot] yarinichatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg   Pavlova Gera junior research fellow   gera [dot] pavlovaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Galimova Juliya research assistant   pertsevaatmcb [dot] nsc [dot] ru
  scopus_logo.jpg   Razuvaeva Alena research assistant   alena [dot] razuvaevaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Popova Julia research assistant   popovaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg   Letiagina Anna research assistant   leschenko [dot] annaatmcb [dot] nsc [dot] ru
      Lepekhina Tatiana lab technician    
  scopus_logo.jpg   Yushkova Anastassiya lab technician   a [dot] yushkovaatmcb [dot] nsc [dot] ru
thomson_logo.gif scopus_logo.jpg Kozhevnikova Elena part-time junior research fellow PhD (Biology)  
thomson_logo.gif scopus_logo.jpg Ivankin Anton part-time research assistant   anton [dot] ivankinatgmail [dot] com
      Korneyeva Lyubov part-time lab technician    

Former research workers:

Anders (Munzarova) Alina
Ivankina (Zotkevich) Elena
Kiseleva Elena, PhD
Lebedev Mikhail
Lebedeva Lidia, DSc
Maltseva Svetlana
Oshchepkova (Alekseyeva) Anastasia
Strunov Anton, PhD

BDGP_logo.jpg

The staff of the Laboratory are among the contributors of Release 6 reference sequence of the Drosophila melanogaster genome

Recent publications: 
  1. Pavlova GA, Popova JV, Andreyeva EN, Yarinich LA, Lebedev MO, Razuvaeva AV, Dubatolova TD, Oshchepkova AL, Pellacani C, Somma MP, Pindyurin AV, Gatti M. RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis. (doi: 10.1371/journal.pgen.1008371PLoS Genet 15(9): e1008371, 2019

  2. Omelina ES, Ivankin AV, Letiagina AE, Pindyurin AV. Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries. (doi: 10.1186/s12864-019-5847-2BMC Genomics 20 (Suppl 7): 536, 2019

  3. Pavlova GA, Razuvaeva AV, Popova JV, Andreyeva EN, Yarinich LA, Lebedev MO, Pellacani C, Bonaccorsi S, Somma MP, Gatti M, Pindyurin AV. The role of Patronin in Drosophila mitosis. (doi: 10.1186/s12860-019-0189-0BMC Mol Cell Biol 20 (Suppl 1): 7, 2019

  4. Gisler S, Gonçalves JP, Akhtar W, de Jong J, Pindyurin AV, Wessels LFA, van Lohuizen M. Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency. (doi: 10.1038/s41467-019-09551-wNature Communications 10: 1598, 2019

  5. Omelina ES, Pindyurin AV. Optogenetic regulation of endogenous gene transcription in mammals. (doi: 10.18699/VJ19.485) Vavilov J Genetics Breeding 23(2): 219-225, 2019

  6. Lebedev MO, Yarinich LA, Ivankin AV, Pindyurin AV. Generation of barcoded plasmid libraries for massively parallel analysis of chromatin position effects. (doi: 10.18699/VJ19.483) Vavilov J Genetics Breeding 23(2): 203-211, 2019

  7. Andreyeva EN, Ogienko AA, Yushkova AA, Popova JV, Pavlova GA, Kozhevnikova EN, Ivankin AV, Gatti M, Pindyurin AV. Non3 is an essential Drosophila gene required for proper nucleolus assembly. (doi: 10.18699/VJ19.481) Vavilov J Genetics Breeding 23(2): 190-198, 2019

  8. Andreyeva EN, Ogienko AA, Dubatolova TD, Oshchepkova AL, Kozhevnikova EN, Ivankin AV, Pavlova GA, Kopyl SA, Pindyurin AV. A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism. (doi: 10.1186/s12863-019-0726-zBMC Genetics 20(Suppl 1): 31, 2019

  9. Fedorova EV, Dorogova NV, Bolobolova EU, Fedorova SA, Karagodin DA, Ogienko AA, Khruscheva AS, Baricheva EM. GAGA protein is required for multiple aspects of Drosophila oogenesis and female fertility. (doi: 10.1002/dvg.23269) Genesis 57(2): e23269, 2019 

  10. Ogienko AG, Markov AV, Sen'kova AV, Logashenko EB, Salomatina OV, Myz SA, Ogienko AA, Nefedov AA, Losev EA, Drebushchak TN, Salakhutdinov NF, Boldyrev VV, Vlasov VV, Zenkova MA, Boldyreva EV. Increasing bioavailability of very poorly water-soluble compounds. A case study of an anti-tumor drug, soloxolon methyl. (doi: 10.1016/j.jddst.2018.10.025J Drug Delivery Sci Tech 49: 35-42, 2019

  11. Pindyurin AV, Ilyin AA, Ivankin AV, Tselebrovsky MV, Nenasheva VV, Mikhaleva EA, Pagie L, van Steensel B, Shevelyov YY. The large fraction of heterochromatin in Drosophila neurons is bound by both B-type lamin and HP1a. (doi: 10.1186/s13072-018-0235-8Epigenetics Chromatin 11: 65, 2018

  12. Ogienko AA, Yarinich LA, Fedorova EV, Lebedev MO, Andreyeva EN, Pindyurin AV, Baricheva EM. New slbo-Gal4 driver lines for the analysis of border cell migration during Drosophila oogenesis. (doi: 10.1007/s00412-018-0676-7) Chromosoma 127(4): 475–487, 2018

  13. Ogienko AG, Myz SA, Ogienko AA, Nefedov AA, Stoporev AS, Mel'gunov MS, Yunoshev AS, Shakhtshneider TP, Boldyrev VV, Boldyreva EV. Cryosynthesis of co-crystals of poorly water-soluble pharmaceutical compounds and their solid dispersions with polymers. The "meloxicam-succinic acid" system as a case study. (doi: 10.1021/acs.cgd.8b01070Crystal Growth Design 18(12): 7401-7409, 2018

  14. Redchuk TA, Karasev MM, Omelina ES, Verkhusha VV. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. (doi: 10.1002/cbic.201700642Chembiochem 19(12): 1334-1340, 2018

  15. Strunov A, Boldyreva LV, Andreyeva EN, Pavlova GA, Popova JV, Razuvaeva AV, Anders AF, Renda F, Pindyurin AV, Gatti M, Kiseleva E. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. (doi: 10.1186/s12915-018-0528-1BMC Biol 16: 68, 2018

  16. La Fortezza M, Grigolon G, Cosolo A, Pindyurin A, Breimann L, Blum H, van Steensel B, Classen A-K. DamID profiling of dynamic Polycomb-binding sites in Drosophila imaginal disc development and tumorigenesis. (doi: 10.1186/s13072-018-0196-yEpigenetics Chromatin 11: 27, 2018

  17. Ogienko AG, Bogdanova EG, Stoporev AS, Ogienko AA, Shinkorenko MP, Yunoshev AS, Manakov AY. Preparation of fine powders by clathrate-forming freeze-drying: a case study of ammonium nitrate. (doi: 10.1016/j.mencom.2018.03.035Mendeleev Communications 28(2): 211-213, 2018

  18. Kozhevnikova EN, Leshchenko AE, Pindyurin AV. An inducible DamID system for profiling interactions of nuclear lamina protein component Lamin B1 with chromosomes in mouse cells. (doi: 10.1134/S0006297918050115) Biochemistry (Mosc) 83(5): 586-594, 2018

  19. Gurova OA, Dubatolova TD, Shlyakhova EV, Omelyanchuk LV, Okotrub AV. Hyperthermal effect of infrared irradiation on aqueous dispersion of carbon nanotubes and their penetration into Drosophila melanogaster larvae. (doi: 10.1002/pssb.201700264) Phys Status Solidi B 255(1): 1700264, 2018

  20. Pindyurin AV. Genome-wide cell type-specific mapping of in vivo chromatin protein binding using an FLP-inducible DamID system in DrosophilaFunctional Genomics. Methods and Protocols. (eds. M Kaufmann, C Klinger, F Savelsbergh). 3rd ed. 392 p. Humana Press, New York, USA. Series volume 1654, 2017. pp. 99-124 (doi: 10.1007/978-1-4939-7231-9_7)

  21. Ilyin AA, Ryazansky SS, Doronin SA, Olenkina OM, Mikhaleva EA, Yakushev EY, Abramov YA, Belyakin SN, Ivankin AV, Pindyurin AV, Gvozdev VA, Klenov MS, Shevelyov YY. Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. (doi: 10.1093/nar/gkx355Nucleic Acids Res 45(13): 7666-7680, 2017

  22. Renda F, Pellacani C, Strunov A, Bucciarelli E, Naim V, Bosso G, Kiseleva E, Bonaccorsi S, Sharp DJ, Khodjakov A, Gatti M, Somma MP. The Drosophila orthologue of the INT6 oncoprotein regulates mitotic microtubule growth and kinetochore structure. (doi: 10.1371/journal.pgen.1006784PLoS Genet 13(5): e1006784, 2017

  23. Andreyeva EN, Bernardo TJ, Kolesnikova TD, Lu X, Yarinich LA, Bartholdy BA, Guo X, Posukh OV, Healton S, Willcockson MA, Pindyurin AV, Zhimulev IF, Skoultchi AI, Fyodorov DV. Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication in Drosophila. (doi: 10.1101/gad.295717.116Genes Dev 31: 603-616, 2017

  24. Omelyanchuk LV, Munzarova AF. Theoretical model of mitotic spindle microtubule growth for FRAP curve interpretation. BMC Syst Biol 11(Suppl 1): 3, 2017

  25. Boldyreva LV, Goncharov FP, Demakova OV, Zykova TYu, Levitsky VG, Kolesnikov NN, Pindyurin AV, Semeshin VF, Zhimulev IF. Protein and genetic composition of four chromatin types in Drosophila melanogaster cell lines. (doi:  10.2174/1389202917666160512164913Curr Genomics 18(2): 214-226, 2017

  26. Renda F, Pellacani C, Strunov A, Bucciarelli E, Naim V, Bosso G, Kiseleva E, Bonaccorsi S, Sharp DJ, Khodjakov A, Gatti M, Somma MP. The Drosophila orthologue of the INT6 onco-protein regulates mitotic microtubule dynamics and kinetochore structure. (doi: 10.1091/mbc.E16-10-0736Mol Biol Cell 27(25): 3947 (P204), 2016

  27. Strunov A, Boldyreva LV, Pavlova GA, Pindyurin AV, Gatti M, Kiseleva E. A simple and effective method for ultrastructural analysis of mitosis in Drosophila S2 cells. (doi: 10.1016/j.mex.2016.10.003MethodsX 3: 551-559, 2016

  28. Munzarova A, Popova J, Razuvaeva A, Shloma V, Gatti M, Omelyanchuk L. Accurate measurement of poleward microtubule flux in the spindle of Drosophila S2 cells. (doi: 10.1002/cbin.10638Cell Biol Int 40: 984-990, 2016

  29. Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van Steensel B. Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila. (doi: 10.1093/nar/gkw176Nucleic Acids Res 44: 5646-5657, 2016

  30. Andreyeva EN, Pechkovsky EV, Pindyurin AV, Gatti M. Pathways of spindle formation in Drosophila mitotic and meiotic cells. Цитология 58: 295-298, 2016

  31. Pavlova GA, Galimova YuA, Popova YuV, Munzarova AF, Razuvaeva AV, Alekseeva AL, Berkaeva MB, Pindyurin AV, Somma MP, Gatti M, Renda F. Factors governing the pattern of spindle microtubule regrowth after tubulin depolymerization. Цитология 58: 299-303, 2016

  32. Pindyurin AV, Gatti M. Chromosomes in the taiga. (doi: 10.1007/s10577-015-9498-0Chromosome Res 23: 641-647, 2015

  33. Zhimulev IF, Zykova TYu, Goncharov FP, Khoroshko VA, Demakova OV, Semeshin VF, Pokholkova GV, Boldyreva LV, Demidova DS, Levitsky VG, Demakov SA, Belyaeva ES. Genetic organization of interphase chromosome bands and interbands in Drosophila melanogaster. (doi: 10.1007/s10577-014-9447-3) Chromosome Res 23: 409-410, 2015

  34. Pindyurin AV, de Jong J, Akhtar W. TRIP through the chromatin: a high throughput exploration of enhancer regulatory landscapes. (doi: 10.1016/j.ygeno.2015.06.009Genomics 106: 171–177, 2015

  35. Yarinich LA, Burakova EA, Zakharov BA, Boldyreva EV, Babkina IN, Tikunova NV, Silnikov VN. Synthesis and structure-activity relationship of novel 1,4-Diazabicyclo[2.2.2]octane derivatives as potent antimicrobial agents. (doi: 10.1016/j.ejmech.2015.03.033Eur J Med Chem 95: 563–573, 2015

  36. Hoskins RA,... Demakova OV, Andreyeva EN, Boldyreva LV,... Zhimulev IF,... Celniker SE. The Release 6 reference sequence of the Drosophila melanogaster genome. (doi: 10.1101/gr.185579.114Genome Res 25: 445-458, 2015

  37. Pokholkova GV, Koryakov DE, Pindyurin AV, Kozhevnikova EN, Belyakin SN, Andreyenkov OV, Belyaeva ES, Zhimulev IF. Tethering of SUUR and HP1 proteins results in delayed replication of euchromatic regions in Drosophila melanogaster polytene chromosomes. (doi: 10.1007/s00412-014-0491-8Chromosoma 124: 209-220, 2015

  38. Bgatova N, Dubatolova T, Omelyanchuk L, Plyusnina E, Shaposhnikov M, Moskalev A. Gadd45 expression correlates with age dependent neurodegeneration in Drosophila melanogaster. (doi: 10.1007/s10522-014-9533-0Biogerontology 16: 53-61, 2015

Selected talks: 
  1. Pavlova G. The role of Drosophila NSL complex in centromere/kinetochore assembly. 14th International conference on Drosophila heterochromatin, 9-15 June 2019, Spoleto, Italy

  2. Pindyurin AV. Input of DNA sequences located in the transcription termination region in gene expression level. International Conference “Chromosome – 2018”, 20-24 August 2018, Novosibirsk, Russia

  3. Ogienko AA. The influence of genetic background in Drosophila fly lines on results on example of line №6458 from Bloomington Drosophila Stock Center. International Conference “Chromosome – 2018”, 20-24 August 2018, Novosibirsk, Russia

  4. Pavlova GA. Roles of Drosophila NSL complex components in mitosis. International Conference “Chromosome – 2018”, 20-24 August 2018, Novosibirsk, Russia

  5. Popova JV. The moonlighting functions of the NON3 protein in Drosophila melanogaster. International Conference “Chromosome – 2018”, 20-24 August 2018, Novosibirsk, Russia

  6. Andreyeva EN. The CG17337 protein is involved in mitosis progression and control the cell death in Drosophila melanogaster. International Conference “Chromosome – 2018”, 20-24 August 2018, Novosibirsk, Russia

  7. Andreyeva E. Functional characterization of the conservative protein CG17337 in Drosophila melanogaster. International mini-conference “Chromosomes and mitosis – 2017”, December 8, 2017, Novosibirsk

  8. Pindyurin A. Large fraction of heterochromatin in Drosophila neurons is simultaneously bound by B-type lamin and HP1a. International mini-conference “Chromosomes and mitosis – 2017”, December 8, 2017, Novosibirsk

  9. Pindyurin A. Efficient delivery of transgenes to cultured Drosophila cells. 13th International conference on Drosophila heterochromatin, June 4-10, 2017, Cagliari, Italy

  10. Pindyurin AV. Cell type-specific mapping of genomic binding sites of chromatin proteins using FLP-inducible DamID systems in Drosophila. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  11. Boldyreva LV. A point deletion in a region immediately downstream of GFP reporter 3′ UTR increases level of GFP transcription in mouse and human cells two-fold. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  12. Yarinich LA. Adaptation of the piggyBac transposon system to study position effects in cultured Drosophila cells. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  13. Munzarova AF. Measuring microtubule flux using the modification of a standard photobleaching-based method. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  14. Razuvaeva AV. Analysis of the involvement of kinesin-like proteins in cell division of Drosophila S2 cells. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  15. Popova JV. Role of Eb1, Mars, Mast and Mei-38 proteins in kinetochore-driven microtubule growth in Drosophila S2 cells. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  16. Pavlova GA. Role of the minus-end binding proteins in kinetochore-driven microtubule growth. International mini-conference "Chromosomes and Mitosis – 2016", November 25, 2016, Novosibirsk

  17. Pindyurin AV. A TRIP through the genome: a high-throughput method to study the influence of chromatin context on gene regulation. 10th Dutch Chromatin Meeting. 24 October 2012, Amsterdam, Netherlands

  18. Andreyeva EN. The SUUR protein affects late replicating chromatin domains in Drosophila melanogaster. Meeting on Eukaryotic DNA replication and genome maintenance, 5-9 September 2007, Cold Spring Harbor, New York, USA

Collaboration: 

  • Università di Roma “Sapienza”, Roma, Italy 

  • The Netherlands Cancer Institute, Amsterdam, Netherlands

  • Ludwig-Maximilians-University Munich, Germany

  • Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

  • Albert Einstein College of Medicine, New York, NY, USA

  • Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA 

  • Massachusetts Institute of Technology, Cambridge, MA, USA