Пиндюрин Алексей Валерьевич - Curriculum Vitae (13th of November 2021)

Alexey V. Pindyurin, PhD

Group leader
e-mail:
a [dot] pindyurinatmcb [dot] nsc [dot] ru
aleksey [dot] pindyurinatgmail [dot] com
 

Work address


Laboratory of Cell Division
Institute of Molecular and Cellular Biology
Lavrentyev ave. 8/2
Novosibirsk
630090
Russia
 

Date and place of birth


31.08.1976 Novosibirsk, Russia
 

Education


1993-1998 Master degree in Biology
Novosibirsk State University
Pirogova str. 2
Novosibirsk 630090
Russia
 
2003-2007 PhD degree in Biology
Institute of Cytology and Genetics
Lavrentyev ave. 10
Novosibirsk 630090
Russia
 

Research experience


1998-2000 Graduate student
Institute of Cytology and Genetics
Novosibirsk, Russia
Supervisor: Dr EM Baricheva (barichatbionet [dot] nsc [dot] ru)
 
2000-2003
Graduate student
University of Geneva
Geneva, Switzerland
Supervisor: Prof V Pirrotta (pirrottaatbiology [dot] rutgers [dot] edu)
 
2003-2007 Research associate
Institute of Cytology and Genetics
Novosibirsk, Russia
Supervisor: Prof IF Zhimulev (zhimulevatmcb [dot] nsc [dot] ru)
 
2008-2013 Post-doc
Netherlands Cancer Institute
Amsterdam, the Netherlands
Supervisor: Prof B van Steensel (b [dot] v [dot] steenselatnki [dot] nl)
 
2013-2014 Post-doc
Institute of Molecular and Cellular Biology
Novosibirsk, Russia
Supervisor: Dr SN Belyakin (belyakinatmcb [dot] nsc [dot] ru)
 
2014-present
Group leader
Institute of Molecular and Cellular Biology
Novosibirsk, Russia

Other experience (selected)


2004
1 month
Visiting scientist
Laboratory of Prof VA Gvozdev (gvozdevatimg [dot] ras [dot] ru)
Institute of Molecular Genetics, Moscow, Russia
 
2004-2005
5 months
Visiting scientist
Laboratory of Prof F Kafatos (f [dot] kafatosatimperial [dot] ac [dot] uk)
European Molecular Biology Laboratory, Heidelberg, Germany
 
2005
2 weeks
Visiting scientist
Laboratory of Prof PG Georgiev (georgiev_patmail [dot] ru)
Institute of Gene Biology, Moscow, Russia
 
2005
1 month
Visiting scientist
Laboratory of Dr B van Steensel (b [dot] v [dot] steenselatnki [dot] nl)
Netherlands Cancer Institute, Amsterdam, the Netherlands
 
2006
3 months
Visiting scientist
Laboratories of Prof F Kafatos (f [dot] kafatosatimperial [dot] ac [dot] uk) and
Dr G Christophides (g [dot] christophidesatimperial [dot] ac [dot] uk)
Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom
 
2007
2.5 months
Visiting scientist
Laboratory of Prof CP Verrijzer (c [dot] verrijzeraterasmusmc [dot] nl)
Erasmus University Medical Centre, Rotterdam, The Netherlands
 

Area of expertise


  • Standard molecular cloning techniques. Southern blot analysis, Northern blot analysis, RT-qPCR analysis
  • Production of recombinant proteins in bacteria, SDS-PAGE, Western blot analysis
  • In vivo formaldehyde DNA-protein cross-linking and immunoprecipitation technique (ChIP). Identification of in vivo DNA targets of chromatin proteins using tethered Dam methyltransferase (DamID)
  • Analysis of DNA-protein and protein-protein interactions (in vitro band shift assay, yeast one- and two-hybrid systems)
  • DNA microarray hybridizations and subsequent data analysis
  • Preparation of samples for Illumina high-throughput sequencing and the subsequent data analysis
  • Thousands of Reporters Integrated in Parallel (TRIP), Massive Parallel Reporter Assay (MPRA) approaches
  • “R”, a language and environment for statistical computing and graphics
  • Drosophila and mammalian (including embryonic stem) cell culture handling. Production of lentiviruses
  • Fly handling and crosses. Drosophila germline transformation
     

Area of interest


Regulation of gene expression, gene silencing, Polycomb and heterochromatin complexes, 3D architecture of chromatin
 

Awards and Grants


2021-2023 Executor
Grant from the Ministry of Science and Higher Education of the Russian Federation 075-15-2021-1086
“Technological platform for the emergency establishment of tools for the prevention and therapy of viral infections on the example of SARS-CoV-2 neutralizing antibodies”
 
2020-2021 Executor
Stiftelsen för internationalisering av högre utbildning och forskning (STINT; the Swedish Foundation for International Cooperation in Research and Higher Education) grant IB2020-8638
“Thousands of Reporters Integrated in Parallel to explore epigenetic regulation by Polycomb group proteins”
 
2019-2020 Supervisor
Russian Science Foundation (RSF) grant 16-14-10288 (extension)
“Multiplex analysis of contribution of chromatin position effect and transcriptional termination on gene expression in eukaryotes”
рнф.рф/project/19-14-13031/
 
2016-2018 Supervisor
Russian Science Foundation (RSF) grant 16-14-10288
“Multiplex analysis of contribution of chromatin position effect and transcriptional termination on gene expression in eukaryotes”
рнф.рф/project/16-14-10288/
 
2016-2018 Supervisor
Russian Foundation for Basic Research (RFBR) grant 16-04-01598 А
“Characterization of functions of highly conservative CG17337 protein associated with replication fork progression inhibitor SUUR in Drosophila melanogaster
 
2016-2018 Executor
Russian Foundation for Basic Research (RFBR) grant 16-04-01018 А
“Studying the mechanisms of tissue- and stage-specific activity of transcription factor GAGA”
 
2015-2017 Executor
Russian Foundation for Basic Research (RFBR) grant 15-04-07653 А
“Study of gut microbiota action on inflammation in experimental models of colitis and bacterial infection”
 
2014-2016 Executor
Mega-grant from the Government of the Russian Federation 14.Z50.31.0005
“The mechanisms of kinetochore-driven microtubule formation in Drosophila
 
2009 The European Academy (Academia Europaea) Prize for young Russian scientists
 
2007 EMBO Short-Term Fellowship #ASTF 238-2007
 
2004 EMBO Short-Term Fellowship #ASTF 267-2004
 

Supervision of PhD students


2020-present Anastasia A Yushkova
preliminary title of the PhD thesis: “Genome-wide analysis of Polycomb-mediated epigenetic regulation in cultured Drosophila and human cells”
 
2017-present Anna E Letiagina
preliminary title of the PhD thesis: “Multiplex analysis of the effects of the transcription termination process on gene activity in eukaryotes”
 
2014-2019 Alyona V Razuvaeva
PhD thesis title: “The role of the Patronin and Asp proteins in the mitotic spindle formation in Drosophila melanogaster
 
2014-2019 Julia V Popova
PhD thesis title: “The role of Eb1, Mars, Non3, Mei-38 and Mast proteins in kinetochore-dependent formation of spindle microtubules in Drosophila melanogaster S2 cell culture”
 

Participation in the organization of international conferences


2019-2021 Competition committee member
“OpenBio” conference, the section “Molecular biology”
Koltsovo, Novosibirsk region, Russia
openbio.ru/en/
 
2015-2019 Organizing committee member
“Chromosomes & Mitosis” mini-conference
Novosibirsk, Russia
www.mcb.nsc.ru/seminars-conference/mini-conference
 
2015 Organizing committee member
“Chromosome 2015” conference
Novosibirsk, Russia
chromosome2015.mcb.nsc.ru/ru/conference.php
 

Participation in the editorial boards of scientific journals


2021-present Review Editor
The "Genome Organization and Dynamics" section within "Frontiers in Molecular Biosciences" journal
 
2020-present Topic co-editor
Research Topic “The Role of High-Order Chromatin Organization in Gene Regulation
The Epigenomics and Epigenetics Section within "Frontiers in Genetics" and "Frontiers in Cell and Developmental Biology" journals
 

Presentations (selected)


2018 International Conference “Chromosome 2018”, Novosibirsk, Russia, 20-24 August

2017 International mini-conference “Chromosomes and mitosis – 2017”, Novosibirsk, Russia, 8 December

2017 13th International conference on Drosophila heterochromatin, Cagliari, Italy, 4-10 June

2016 International mini-conference “Chromosomes and Mitosis – 2016”, Novosibirsk, Russia, 25 November

2015 The 8th Russian Congress of Young Biologists Symbiose – Russia 2015, Novosibirsk, Russia, 5-9 October

2014 Scientific School on molecular and cellular biology, Novosibirsk, Russia, 3-5 September

2014 International Conference (Living cells study (LCS) programme) “Chromatin meeting 2014”, Novosibirsk, Russia, 11 March

2012 10th Dutch Chromatin Meeting, Amsterdam, The Netherlands, 24 October

2012 International Conference “Chromosome 2012”, Novosibirsk, Russia, 2-7 September
 

List of publications


PubMed

Google Scholar

Publons (ResearcherID G-4167-2014)

Scopus ID 6504141292

ORCID

  1. Katokhin AV, Pindyurin AV, Fedorova EV, Baricheva EM. Molecular genetic analysis of the Drosophila melanogaster Trithorax-like gene coding for the GAGA transcription factor.
    Russian Journal of Genetics. 2001. 37(4): 467-474
    DOI: 10.1023/A:1016694124303
    https://link.springer.com/article/10.1023/A:1016694124303
     
  2. Kolesnikova TD, Andreeva EN, Pindyurin AV, Ananko NG, Belyakin SN, Shloma VV, Yurlova AA, Makunin IV, Pokholkova GV, Volkova EI, Zarutskaya EA, Kokoza EB, Semeshin VF, Belyaeva ES, Zhimulev IF. Contribution of the SuUR gene to the organization of epigenetically repressed regions of Drosophila melanogaster chromosomes.
    Russian Journal of Genetics. 2006. 42(8): 831-844
    DOI: 10.1134/S1022795406080011
    https://link.springer.com/article/10.1134/S1022795406080011
     
  3. Pindyurin AV, Moorman C, de Wit E, Belyakin SN, Belyaeva ES, Christophides GK, Kafatos FC, van Steensel B, Zhimulev IF. SUUR joins separate subsets of PcG, HP1 and B-type lamin targets in Drosophila.
    Journal of Cell Science. 2007. 120(14): 2344-2351
    DOI: 10.1242/jcs.006007
    http://jcs.biologists.org/content/120/14/2344.long
     
  4. Pindyurin AV, Boldyreva LV, Shloma VV, Kolesnikova TD, Pokholkova GV, Andreyeva EN, Kozhevnikova EN, Ivanoschuk IG, Zarutskaya EA, Demakov SA, Gorchakov AA, Belyaeva ES, Zhimulev IF. Interaction between the Drosophila heterochromatin proteins SUUR and HP1.
    Journal of Cell Science. 2008. 121(10): 1693-1703
    DOI: 10.1242/jcs.018655
    http://jcs.biologists.org/content/121/10/1693.long
     
  5. Zhimulev IF, Belyaeva ES, Andreenkova NG, Andreeva EN, Belyakin SN, Boldyreva LV, Brusentsova IV, Volkova EI, Demakov SA, Demakova OV, Zarutskaya EA, Zykov IA, Kokoza EB, Kolesnikova TD, Komor UA, Koryakov DE, Makunin IV, Pindyurin AV, Pokholkova GV, Semeshin VF, Shloma VV, Yurlova AA. SuUR gene – a unique instrument for the study of structure and organization of Drosophila chromosomes and genome.
    VOGiS Herald. 2008. 12(1/2): 127-148 (in Russian)
    https://elibrary.ru/item.asp?id=12515811
     
  6. Andreyenkova NG, Kokoza EB, Semeshin VF, Belyaeva ES, Demakov SA, Pindyurin AV, Andreyeva EN, Volkova EI, Zhimulev IF. Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes.
    Chromosoma. 2009. 118(6): 747-761
    DOI: 10.1007/s00412-009-0232-6
    https://link.springer.com/article/10.1007%2Fs00412-009-0232-6
     
  7. Fedorova EV, Pindyurin AV, Baricheva EM. Maintenance of the patterns of expression of homeotic genes in the development of Drosophila melanogaster by proteins of the Polycomb, trithorax, and ETP Groups.
    Russian Journal of Genetics. 2009. 45(10): 1145-1161
    DOI: 10.1134/S1022795409100019
    https://link.springer.com/article/10.1134/S1022795409100019
     
  8. Zhimulev IF, Belyaeva ES, Andreeva EN, Andreenkova NG, Babenko VN, Belyakin SN, Boldyreva LV, Brusentsova IV, Demakov SA, Demakova OV, Zykov IA, Kokoza EB, Kolesnikova TD, Maksimov DA, Makunin IV, Pindyurin AV, Semeshin VF, Khoroshko VA. Intercalary Heterochromatin in the Genome of Drosophila.
    Russian Journal of Genetics. 2010. 46(10): 1240-1242
    DOI: 10.1134/S1022795410100248
    https://link.springer.com/article/10.1134/S1022795410100248
     
  9. Pindyurin AV, van Steensel B. Hox in space: Gene cluster regulation linked to folding of chromatin.
    Nucleus. 2012. 3(2): 118-122
    DOI: 10.4161/nucl.19159
    http://www.tandfonline.com/doi/full/10.4161/nucl.19159
     
  10. Kozhevnikova EN, van der Knaap JA, Pindyurin AV, Ozgur Z, van Ijcken WF, Moshkin YM, Verrijzer CP. Metabolic enzyme IMPDH is also a transcription factor regulated by cellular state
    Molecular Cell. 2012. 47(1): 133-139
    DOI: 10.1016/j.molcel.2012.04.030
    https://www.sciencedirect.com/science/article/pii/S1097276512003528
     
  11. Akhtar W*, de Jong J*, Pindyurin AV*, Pagie L, Meuleman W, de Ridder J, Berns A, Wessels LF, van Lohuizen M, van Steensel B. Chromatin position effects assayed by thousands of reporters integrated in parallel.
    Cell. 2013. 154(4): 914-927
    DOI: 10.1016/j.cell.2013.07.018 (*equal contribution) 
    https://www.sciencedirect.com/science/article/pii/S0092867413008891
     
  12. Akhtar W*, Pindyurin AV*, de Jong J, Pagie L, ten Hoeve J, Berns A, Wessels LF, van Steensel B, van Lohuizen M. Using TRIP for genome-wide position effect analysis in cultured cells.
    Nature Protocols. 2014. 9(6): 1255-1281
    DOI: 10.1038/nprot.2014.072 (*equal contribution) 
    https://www.nature.com/articles/nprot.2014.072
     
  13. Nordman JT, Kozhevnikova EN, Verrijzer CP, Pindyurin AV, Andreyeva EN, Shloma VV, Zhimulev IF, Orr-Weaver TL. DNA copy number control through inhibition of replication fork progression.
    Cell Reports. 2014. 9(3): 841-849
    DOI: 10.1016/j.celrep.2014.10.005
    https://www.sciencedirect.com/science/article/pii/S2211124714008596
     
  14. Pokholkova GV, Koryakov DE, Pindyurin AV, Kozhevnikova EN, Belyakin SN, Andreyenkov OV, Belyaeva ES, Zhimulev IF. Tethering of SUUR and HP1 proteins results in delayed replication of euchromatic regions in Drosophila melanogaster polytene chromosomes.
    Chromosoma. 2015. 124(2): 209-220
    DOI: 10.1007/s00412-014-0491-8
    https://link.springer.com/article/10.1007%2Fs00412-014-0491-8
     
  15. Pindyurin AV, de Jong J, Akhtar W. TRIP through the chromatin: A high throughput exploration of enhancer regulatory landscapes.
    Genomics. 2015. 106(3): 171–177
    DOI: 10.1016/j.ygeno.2015.06.009
    http://www.sciencedirect.com/science/article/pii/S0888754315300136 
     
  16. Pindyurin AV, Gatti M. Chromosomes in the taiga.
    Chromosome Research. 2015. 23(4): 641-647
    DOI: 10.1007/s10577-015-9498-0
    https://link.springer.com/article/10.1007%2Fs10577-015-9498-0
     
  17. Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van Steensel B. Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila.
    Nucleic Acids Research. 2016. 44(12): 5646-5657
    DOI: 10.1093/nar/gkw176
    https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw176
     
  18. Pavlova GA, Galimova JA, Popova JV, Munzarova AF, Razuvaeva AV, Alekseeva AL, Berkaeva MB, Pindyurin AV, Somma MP, Gatti M, Renda F. Factors governing the pattern of spindle microtubule regrowth after tubulin depolymerization.
    Tsitologiya. 2016. 58(4): 299-303
    http://www.tsitologiya.cytspb.rssi.ru/58_4/pavlova.pdf
     
  19. Andreyeva EN, Pechkovsky EV, Pindyurin AV, Gatti M. Pathways of spindle formation in Drosophila mitotic and meiotic cells.
    Tsitologiya. 2016. 58(4): 295-298
    http://www.tsitologiya.cytspb.rssi.ru/58_4/andreyeva.pdf
     
  20. Strunov A, Boldyreva LV, Pavlova GA, Pindyurin AV, Gatti M, Kiseleva E. A simple and effective method for ultrastructural analysis of mitosis in Drosophila S2 cells.
    MethodsX. 2016. 3: 551-559
    DOI: 10.1016/j.mex.2016.10.003
    https://www.sciencedirect.com/science/article/pii/S221501611630036X
     
  21. Boldyreva LV, Goncharov FP, Demakova OV, Zykova TY, Levitsky VG, Kolesnikov NN, Pindyurin AV, Semeshin VF, Zhimulev IF. Protein and genetic composition of four chromatin types in Drosophila melanogaster cell lines.
    Current Genomics. 2017. 18(2): 214-226
    DOI: 10.2174/1389202917666160512164913
    http://www.eurekaselect.com/142119
     
  22. Pindyurin AV. Genomic mapping of chromatin proteins by using Daminv modification of an FLP-dependent DamID approach.
    Doklady Biochemistry and Biophysics. 2017. 472(1): 15-18
    DOI: 10.1134/S1607672917010057
    https://link.springer.com/article/10.1134%2FS1607672917010057
     
  23. Andreyeva EN, Bernardo TJ, Kolesnikova TD, Lu X, Yarinich LA, Bartholdy BA, Guo X, Posukh OV, Healton S, Willcockson MA, Pindyurin AV, Zhimulev IF, Skoultchi AI, Fyodorov DV. Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication in Drosophila.
    Genes and Development. 2017. 31(6): 603-616
    DOI: 10.1101/gad.295717.116
    http://genesdev.cshlp.org/content/31/6/603.long
     
  24. Ilyin AA, Ryazansky SS, Doronin SA, Olenkina OM, Mikhaleva EA, Yakushev EY, Abramov YA, Belyakin SN, Ivankin AV, Pindyurin AV, Gvozdev VA, Klenov MS, Shevelyov YY. Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells.
    Nucleic Acids Research. 2017. 45(13): 7666-7680
    DOI: 10.1093/nar/gkx355
    https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkx355
     
  25. Pindyurin AV. Genome-wide cell type-specific mapping of in vivo chromatin protein binding using an FLP-inducible DamID system in Drosophila.
    Methods in Molecular Biology (Chapter in “Functional Genomics”, 3rd edition, eds. M Kaufmann, C Klinger, F Savelsbergh, 392 pages, Humana Press, New York, USA) 2017. 1654: 99-124
    DOI: 10.1007/978-1-4939-7231-9_7
    https://link.springer.com/protocol/10.1007%2F978-1-4939-7231-9_7
     
  26. Zakharenko LP, Petrovskii DV, Dranov IG, Fedorova SA, Yudin NS, Pindyurin AV, Moshkin YM. A link between phenotypic robustness and life expectancy in Drosophila melanogaster.
    Vavilov Journal of Genetics and Breeding. 2017. 21(7): 816-824
    DOI 10.18699/VJ17.301 (in Russian) 
    http://www.bionet.nsc.ru/vogis/download/14_Zakharenko.pdf
     
  27. Kozhevnikova EN, Leshchenko AE, Pindyurin AV. An inducible DamID system for profiling interactions of nuclear lamina protein component Lamin B1 with chromosomes in mouse cells.
    Biochemistry (Mosc). 2018. 83(5): 586-594
    DOI: 10.1134/S0006297918050115
    https://link.springer.com/article/10.1134%2FS0006297918050115
     
  28. La Fortezza M, Grigolon G, Cosolo A, Pindyurin A, Breimann L, Blum H, van Steensel B, Classen A-K. DamID profiling of dynamic Polycomb-binding sites in Drosophila imaginal disc development and tumorigenesis.
    Epigenetics & Chromatin. 2018. 11: 27
    DOI: 10.1186/s13072-018-0196-y
    https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-018-0196-y
     
  29. Strunov A, Boldyreva LV, Andreyeva EN, Pavlova GA, Popova JV, Razuvaeva AV, Anders AF, Renda F, Pindyurin AV, Gatti M, Kiseleva E. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors.
    BMC Biology. 2018. 16: 68
    DOI: 10.1186/s12915-018-0528-1
    https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0528-1
     
  30. Ogienko AA, Yarinich LA, Fedorova EV, Lebedev MO, Andreyeva EN, Pindyurin AV, Baricheva EM. New slbo-Gal4 driver lines for the analysis of border cell migration during Drosophila oogenesis.
    Chromosoma. 2018. 127(4): 475-487
    DOI: 10.1007/s00412-018-0676-7
    https://link.springer.com/article/10.1007%2Fs00412-018-0676-7
     
  31. Pindyurin AV, Ilyin AA, Ivankin AV, Tselebrovsky MV, Nenasheva VV, Mikhaleva EA, Pagie L, van Steensel B, Shevelyov YY. The large fraction of heterochromatin in Drosophila neurons is bound by both B-type lamin and HP1a.
    Epigenetics & Chromatin. 2018. 11(1): 65
    DOI: 10.1186/s13072-018-0235-8
    https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-018-0235-8
     
  32. Andreyeva EN, Ogienko AA, Dubatolova TD, Oshchepkova AL, Kozhevnikova EN, Ivankin AV, Pavlova GA, Kopyl SA, Pindyurin AV. A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism.
    BMC Genetics. 2019. 20(Suppl 1): 31
    DOI: 10.1186/s12863-019-0726-z
    https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-019-0726-z
     
  33. Andreyeva EN, Ogienko AA, Yushkova AA, Popova JV, Pavlova GA, Kozhevnikova EN, Ivankin AV, Gatti M, Pindyurin AV. Non3 is an essential Drosophila gene required for proper nucleolus assembly.
    Vavilov Journal of Genetics and Breeding. 2019. 23(2): 190-198
    DOI: 10.18699/VJ19.481
    https://vavilov.elpub.ru/jour/article/view/1935/1199
     
  34. Lebedev MO, Yarinich LA, Ivankin AV, Pindyurin AV. Generation of barcoded plasmid libraries for massively parallel analysis of chromatin position effects.
    Vavilov Journal of Genetics and Breeding. 2019. 23(2): 203-211
    DOI: 10.18699/VJ19.483
    https://vavilov.elpub.ru/jour/article/view/1937/1201
     
  35. Omelina ES, Pindyurin AV. Optogenetic regulation of endogenous gene transcription in mammals.
    Vavilov Journal of Genetics and Breeding. 2019. 23(2): 219-225
    DOI: 10.18699/VJ19.485
    https://vavilov.elpub.ru/jour/article/view/1939/1203
     
  36. Gisler S, Gonçalves JP, Akhtar W, de Jong J, Pindyurin AV, Wessels LFA, van Lohuizen M. Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency.
    Nature Communications. 2019. 10: 1598
    DOI: 10.1038/s41467-019-09551-w
    https://www.nature.com/articles/s41467-019-09551-w
     
  37. Pavlova GA, Razuvaeva AV, Popova JV, Andreyeva EN, Yarinich LA, Lebedev MO, Pellacani C, Bonaccorsi S, Somma MP, Gatti M, Pindyurin AV. The role of Patronin in Drosophila mitosis.
    BMC Molecular and Cell Biology. 2019. 20(Suppl 1): 7
    DOI: 10.1186/s12860-019-0189-0
    https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/s12860-019-0189-0
     
  38. Omelina ES, Ivankin AV, Letiagina AE, Pindyurin AV. Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries.
    BMC Genomics. 2019. 20(Suppl 7): 536
    DOI: 10.1186/s12864-019-5847-2
    https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5847-2
     
  39. Pavlova GA, Popova JV, Andreyeva EN, Yarinich LA, Lebedev MO, Razuvaeva AV, Dubatolova TD, Oshchepkova AL, Pellacani C, Somma MP, Pindyurin AV, Gatti M. RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.
    PLoS Genetics. 2019. 15(9): e1008371
    DOI: 10.1371/journal.pgen.1008371
    https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008371
     
  40. Borisova MA, Snytnikova OA, Litvinova EA, Achasova KM, Babochkina TI, Pindyurin AV, Tsentalovich YuP, Kozhevnikova EN. Fucose ameliorates tryptophan metabolism and behavioral abnormalities in a mouse model of chronic colitis.
    Nutrients. 2020. 12(2): 445
    DOI: 10.3390/nu12020445
    https://www.mdpi.com/2072-6643/12/2/445
     
  41. Somma MP, Andreyeva EN, Pavlova GA, Pellacani C, Bucciarelli E, Popova JV, Bonaccorsi S, Pindyurin AV, Gatti M. Moonlighting in mitosis: Analysis of the mitotic functions of transcription and splicing factors.
    Cells. 2020. 9: 1554
    DOI: 10.3390/cells9061554
    https://www.mdpi.com/2073-4409/9/6/1554
     
  42. Ogienko AA, Yarinich LA, Fedorova EV, Dorogova NV, Bayborodin SI, Baricheva EM, Pindyurin AV. GAGA regulates border cell migration in Drosophila.
    International Journal of Molecular Sciences. 2020. 21: 7468
    DOI: 10.3390/ijms21207468
    https://www.mdpi.com/1422-0067/21/20/7468
     
  43. Ogienko AA, Andreyeva EN, Omelina ES, Oshchepkova AL, Pindyurin AV. Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology.
    BMC Genetics. 2020. 21(Suppl 1): 96
    DOI: 10.1186/s12863-020-00895-7
    https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-020-00895-7
     
  44. Dolskiy AA, Yarushkin AA, Grishchenko IV, Lemskaya NA, Pindyurin AV, Boldyreva LV, Pustylnyak VO, Yudkin DV. miRNA expression and interaction with the 3′UTR of FMR1 in FRAXopathy pathogenesis.
    Non-coding RNA Research. 2021. 6(1): 1-7
    DOI: 10.1016/j.ncrna.2020.11.006
    https://www.sciencedirect.com/science/article/pii/S2468054020300743
     
  45. Boldyreva LV, Yarinich LA, Kozhevnikova EN, Ivankin AV, Lebedev MO, Pindyurin AV. Fine gene expression regulation by minor sequence variations downstream of the polyadenylation signal.
    Molecular Biology Reports. 2021. 48(2): 1539-1547
    DOI: 10.1007/s11033-021-06160-z
    https://link.springer.com/article/10.1007%2Fs11033-021-06160-z
     
  46. Letiagina AE, Omelina ES, Ivankin AV, Pindyurin AV. MPRAdecoder: Processing of the raw MPRA data with a priori unknown sequences of the region of interest and associated barcodes.
    Frontiers in Genetics. 2021. 12: 618189
    DOI: 10.3389/fgene.2021.618189
    https://www.frontiersin.org/articles/10.3389/fgene.2021.618189/full
     
  47. Boldyreva LV, Andreyeva EN, Pindyurin AV. Gene position effect: Role of the local environment in regulation of gene activity.
    Molecular Biology. 2022
    Accepted for publication